The influence of rearing on behavior, brain monoamines and gene expression in three-spined sticklebacks

SND-ID: 2024-225. Version: 1. DOI: https://doi.org/10.5878/1txx-m695

Citering

Skapare/primärforskare

Robin N Abbey-Lee - Linköpings unviversitet, Institutionen för fysik, kemi och biologi

Emily J Uhrig - Stockholms universitet, Zoologiska institutionen

Josefina Zidar - Linköpings universite, Institutionen för fysik, kemi och biologi

Anna Favati - Stockholms universitet, Zoologiska institutionen

Johan Almberg - Linköpings universitet, Institutionen för fysik, kemi och biologi,

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Robin N Abbey-Lee - Linköpings unviversitet, Institutionen för fysik, kemi och biologi

Emily J Uhrig - Stockholms universitet, Zoologiska institutionen

Josefina Zidar - Linköpings universite, Institutionen för fysik, kemi och biologi

Anna Favati - Stockholms universitet, Zoologiska institutionen

Johan Almberg - Linköpings universitet, Institutionen för fysik, kemi och biologi,

Josefin Dahlblom - Uppsala universitet, Department of Neuroscience

Svante Winberg - Uppsala universitet, Department of Neuroscience

Hanne Løvlie - Linköpings universitet, Institutionen för fysik, kemi och biologi orcid

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Forskningshuvudman

Linköpings universitet rorId

Beskrivning

Data file for the study: The influence of rearing on behavior, brain monoamines, and gene expression in three-spined sticklebacks, RN Abbey-Lee, EJ Uhrig, J Zidar, A Favati, J Almberg, J Dahlblom, S Winberg, H Lovlie.

Corresponding Author: H Lovlie, hanne.lovlie@liu.se

File contents: All data used for the study- including treatment status, behaviour, qPCR gene expression, and HPLC-EC monoamine levels.

The causes of individual variation in behavior are often not well understood, and potential underlying mechanisms include both intrinsic and extrinsic factors, such as early environmental, physiological, and genetic differences.

In an exploratory laboratory study, we raised three-spined sticklebacks (Gasterosteus aculeatus) under 4 different environmental conditions (simulated predator environment, complex environment, variable social environment, and control). We investigated how these manipulations related to behavior, brain physiology and gene expression later in life, with focus on brain dopamine and serotonin levels, turnover rates, and gene expression.

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Data file for the study: The influence of rearing on behavior, brain monoamines, and gene expression in three-spined sticklebacks, RN Abbey-Lee, EJ Uhrig, J Zidar, A Favati, J Almberg, J Dahlblom, S Winberg, H Lovlie.

Corresponding Author: H Lovlie, hanne.lovlie@liu.se

File contents: All data used for the study- including treatment status, behaviour, qPCR gene expression, and HPLC-EC monoamine levels.

The causes of individual variation in behavior are often not well understood, and potential underlying mechanisms include both intrinsic and extrinsic factors, such as early environmental, physiological, and genetic differences.

In an exploratory laboratory study, we raised three-spined sticklebacks (Gasterosteus aculeatus) under 4 different environmental conditions (simulated predator environment, complex environment, variable social environment, and control). We investigated how these manipulations related to behavior, brain physiology and gene expression later in life, with focus on brain dopamine and serotonin levels, turnover rates, and gene expression.

The different rearing environments influenced behavior and gene expression, but did not alter monoamine levels or metabolites. Specifically, compared to control fish, fish exposed to a simulated predator environment tended to be less aggressive, more exploratory, and more neophobic; and fish raised in both complex and variable social environments tended to be less neophobic. Exposure to a simulated predator environment tended to lower expression of dopamine receptor DRD4A, a complex environment increased expression of dopamine receptor DRD1B, while a variable social environment tended to increase serotonin receptor 5-HTR2B and increased serotonin transporter SLC6A4A expression. Despite both behavior and gene expression varying with early environment, there was no evidence that gene expression mediated the relationship between early environment and behavior.

Our results confirm that environmental conditions early in life can affect phenotypic variation. However, the mechanistic pathway of the monoaminergic systems translating early environmental variation into observed behavioral responses was not detected.

Datasetet har ursprungligen publicerats i DiVA och flyttades över till SND 2024. Visa mindre..

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Forskningsområde

Biologi (Standard för svensk indelning av forskningsämnen 2011)

Publikationer

Abbey-Lee, R. N., Uhrig, E., Zidar, J., Favati, A., Almberg, J., Dahlbom, J., Winberg, S., & Løvlie, H. (n.d.). The Influence of Rearing on Behavior, Brain Monoamines, and Gene Expression in Three-Spined Sticklebacks. In Brain, behavior, and evolution (Vol. 91, Issue 4, pp. 201–213). https://doi.org/10.1159/000489942
URN: urn:nbn:se:su:diva-161239
DOI: https://doi.org/10.1159/000489942
SwePub: oai:DiVA.org:su-161239

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Publicerad: 2024-06-26