Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - The molecular chaperone GFP-Hsp104 before and after heat stress in a Hsp70-mutant yeast strain with exogenous complementation of wildtype and chimaeric mutant alleles of yeast Hsp70-alleles
SND-ID: 2020-36-6. Version: 1. DOI: https://doi.org/10.5878/mez1-3z30
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GFP-HSP104 in WT and ssa12DD with SSA1-4 chimaeras
- 180615 WT and ssa1-2DD GFP-HSP104 w. SSA4-SSA1 constructs.zip (11.7 GB)
- 180618 ssa1-2DD GFP-HSP104 w SSA1-SSA4 constructs.zip (12.41 GB)
- 180621 ssa1-2DD GFP-HSP104 w SSA1-SSA4 constructs.zip (12.05 GB)
- 180627 ssa1-2DD GFP-HSP104 w SSA1-SSA4 constructs.zip (11.12 GB)
- 180628 WT and ssa1-2DD GFP-HSP104 w SSA1-SSA4 constructs.zip (10.85 GB)
- 180703 WT and ssa1-2DD GFP-HSP104 w SSA1-SSA4 constructs.zip (19.85 GB)
- 180705 WT and ssa1-2DD GFP-HSP104 w SSA1-SSA4 constructs.zip (17.95 GB)
Associated documentation
2D gel electrophoresis.pdf (330.81 KB)
Data set file index.xlsx (42.92 KB)
GFP-HSP104 in WT and HSP70 mutants.pdf (338.1 KB)
GFP-HSP104 in WT and ssa12DD with SSA1-4 chimaeras.pdf (342.27 KB)
guk1-7-GFP microscopy.pdf (338.56 KB)
guk1-7-GFP w Sik1-RFP and DAPI.pdf (339.77 KB)
guk1-7-GFP w-wo HSP104 microscopy.pdf (341.27 KB)
gus1-3-GFP microscopy.pdf (335.64 KB)
Hsp42 IF microscopy.pdf (536.47 KB)
Mca1-GFP microscopy.pdf (335.98 KB)
... Show more..2D gel electrophoresis.pdf (330.81 KB)
Data set file index.xlsx (42.92 KB)
GFP-HSP104 in WT and HSP70 mutants.pdf (338.1 KB)
GFP-HSP104 in WT and ssa12DD with SSA1-4 chimaeras.pdf (342.27 KB)
guk1-7-GFP microscopy.pdf (338.56 KB)
guk1-7-GFP w Sik1-RFP and DAPI.pdf (339.77 KB)
guk1-7-GFP w-wo HSP104 microscopy.pdf (341.27 KB)
gus1-3-GFP microscopy.pdf (335.64 KB)
Hsp42 IF microscopy.pdf (536.47 KB)
Mca1-GFP microscopy.pdf (335.98 KB)
ReadMe Access to microscopy files.pdf (225.14 KB)
Ssa4-GFP Mca1-RFP microscopy.pdf (510.88 KB)
Timelapse microscopy.pdf (343.15 KB)
Total Hsp70 western blots.pdf (587.73 KB)
Show less..Citation
Creator/Principal investigator(s)
Rebecca Andersson - University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Sarah Hanzén - Cochlear Nordic AB
Katarina Vielfort - Umeå University, Institute of Molecular Biology
Thomas Nyström - University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Research principal
University of Gothenburg - Institute of Biomedicine, Department of Microbiology and Immunology
Description
Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.
Fluorescence microscopy of live yeast cells at mid-exponential growth and after 30 minutes of heat shock. The cells were grown in complete synthetic media lacking histidine and leucine with 2 % galactose as carbon source. All strains carry two plasmids; one expressing GFP-HSP104 under the control of the GAL-promoter and one expressing SSA1, SSA4 or SSA1/SSA4 chimaeras (alternatively an empty
Fluorescence microscopy of live yeast cells at mid-exponential growth and after 30 minutes of heat shock. The cells were grown in complete synthetic media lacking histidine and leucine with 2 % galactose as carbon source. All strains carry two plasmids; one expressing GFP-HSP104 under the control of the GAL-promoter and one expressing SSA1, SSA4 or SSA1/SSA4 chimaeras (alternatively an empty plasmid) under the control of the GPD-promoter.
The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi). Show less..
Data contains personal data
No
Language
Unit of analysis
Population
Saccharomyces cerevisiae (Baker's yeast)
Study design
Experimental study
Preclinical study
Sampling procedure
Time period(s) investigated
2012 – 2020
Data format / data structure
Responsible department/unit
Institute of Biomedicine, Department of Microbiology and Immunology
Research area
Biochemistry and molecular biology (Standard för svensk indelning av forskningsämnen 2011)
Cell biology (Standard för svensk indelning av forskningsämnen 2011)
Microbiology (Standard för svensk indelning av forskningsämnen 2011)
Cell and molecular biology (Standard för svensk indelning av forskningsämnen 2011)
Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full
DOI:
https://doi.org/10.1101/2020.06.25.170670
Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951.
DOI:
https://doi.org/10.1371/journal.pgen.1008951
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