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Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Western blots of SDS-PAGE gels with primary antibodies against Hsp70p and Pgk1p

SND-ID: 2020-36-9. Version: 1. DOI: https://doi.org/10.5878/rq33-3f44

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2020-36-9-1.zip (~5.85 MB)

Citation

Creator/Principal investigator(s)

Rebecca Andersson - University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology orcid

Thomas Nyström - University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology orcid

Research principal

University of Gothenburg - Institute of Biomedicine, Department of Microbiology and Immunology rorId

Description

Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.

WT ssa12DD and ssa12DDSSA4OE: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth. The yeast was grown in complete synthetic media with 2 % glucose as carbon source.
WT ssa12DD w chimaera plasmids: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth before and after 30 minutes of heat shock. The yeast was grown in c

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Our study aims to answer the question "Which functions of the Hsp70 class of molecular chaperones are essential for yeast to maintain a standard replicative life span?". To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones. The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts.

WT ssa12DD and ssa12DDSSA4OE: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth. The yeast was grown in complete synthetic media with 2 % glucose as carbon source.
WT ssa12DD w chimaera plasmids: Western blots of SDS-PAGE gels with protein extracts from yeast in mid-exponential growth before and after 30 minutes of heat shock. The yeast was grown in complete synthetic media lacking leucine with 2 % glucose as carbon source.
The images are scans of western blot PVDF-membranes.
The image files are provided in TIFF format. Show less..

Data contains personal data

No

Language

Method and outcome

Unit of analysis

Population

Saccharomyces cerevisiae (Baker's yeast)

Study design

Experimental study

Preclinical study

Sampling procedure

Time period(s) investigated

2012 – 2020

Data format / data structure

Data collection
  • Mode of collection: Biological tests
  • Time period(s) for data collection: 2018-04-11 – 2018-07-24
  • Data collector: University of Gothenburg
  • Source of the data: Research data: Unpublished, Biological samples, Research data
Geographic coverage
Administrative information

Responsible department/unit

Institute of Biomedicine, Department of Microbiology and Immunology

Funding

  • Funding agency: Knut and Alice Wallenberg Foundation rorId
Topic and keywords

Research area

Biochemistry and molecular biology (Standard för svensk indelning av forskningsämnen 2011)

Cell biology (Standard för svensk indelning av forskningsämnen 2011)

Microbiology (Standard för svensk indelning av forskningsämnen 2011)

Cell and molecular biology (Standard för svensk indelning av forskningsämnen 2011)

Keywords

Molecular chaperones, Hsp70 heat-shock proteins, Proteostasis deficiencies

Publications

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Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full
DOI: https://doi.org/10.1101/2020.06.25.170670

Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951.
DOI: https://doi.org/10.1371/journal.pgen.1008951

If you have published anything based on these data, please notify us with a reference to your publication(s). If you are responsible for the catalogue entry, you can update the metadata/data description in DORIS.

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License

CC BY 4.0

Versions

Version 1. 2020-12-16

Version 1: 2020-12-16

DOI: https://doi.org/10.5878/rq33-3f44

Contact for questions about the data

Related research data in SND's catalogue

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Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Intracellular colocalisation of the chaperone Ssa4-GFP and the metacaspase Mca1-RFP before and after heat stress in a Hsp70-mutant yeast cell strain

Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - The molecular chaperone GFP-Hsp104 before and after heat stress in Hsp70-mutant yeast strains

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Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Hsp70-mutant yeast cells with the misfolding marker protein guk1-7-GFP, the nucleolar marker Sik1-RFP and nuclear staining with DAPI imaged before, directly after, and during recovery from heat stress

Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Hsp70-mutant yeast strains with the misfolding marker protein guk1-7-GFP and with or without an intact HSP104-allele, imaged during mid-exponential growth

Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Immunocytofluoroscense of Hsp70-mutant yeast strains with primary antibodies against Hsp42p

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Published: 2020-12-16
Last updated: 2021-02-11