Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Intracellular colocalisation of the chaperone Ssa4-GFP and the metacaspase Mca1-RFP before and after heat stress in a Hsp70-mutant yeast cell strain
SND-ID: 2020-36-3. Version: 1. DOI: https://doi.org/10.5878/hvf7-3j18
Associated documentation
2D gel electrophoresis.pdf (330.81 KB)
Data set file index.xlsx (42.92 KB)
GFP-HSP104 in WT and HSP70 mutants.pdf (338.1 KB)
GFP-HSP104 in WT and ssa12DD with SSA1-4 chimaeras.pdf (342.27 KB)
guk1-7-GFP microscopy.pdf (338.56 KB)
guk1-7-GFP w Sik1-RFP and DAPI.pdf (339.77 KB)
guk1-7-GFP w-wo HSP104 microscopy.pdf (341.27 KB)
gus1-3-GFP microscopy.pdf (335.64 KB)
Hsp42 IF microscopy.pdf (536.47 KB)
Mca1-GFP microscopy.pdf (335.98 KB)
... Show more..2D gel electrophoresis.pdf (330.81 KB)
Data set file index.xlsx (42.92 KB)
GFP-HSP104 in WT and HSP70 mutants.pdf (338.1 KB)
GFP-HSP104 in WT and ssa12DD with SSA1-4 chimaeras.pdf (342.27 KB)
guk1-7-GFP microscopy.pdf (338.56 KB)
guk1-7-GFP w Sik1-RFP and DAPI.pdf (339.77 KB)
guk1-7-GFP w-wo HSP104 microscopy.pdf (341.27 KB)
gus1-3-GFP microscopy.pdf (335.64 KB)
Hsp42 IF microscopy.pdf (536.47 KB)
Mca1-GFP microscopy.pdf (335.98 KB)
ReadMe Access to microscopy files.pdf (225.14 KB)
Ssa4-GFP Mca1-RFP microscopy.pdf (510.88 KB)
Timelapse microscopy.pdf (343.15 KB)
Total Hsp70 western blots.pdf (587.73 KB)
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Citation
Creator/Principal investigator(s)
Katarina Vielfort - Umeå University, Institute of Molecular Biology
Rebecca Andersson - University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Thomas Nyström - University of Gothenburg, Institute of Biomedicine, Department of Microbiology and Immunology
Research principal
University of Gothenburg - Institute of Biomedicine, Department of Microbiology and Immunology
Description
Fluorescence microscopy of live yeast cells at mid-exponential growth before and after 30 minutes of heat shock. All strains produce the Hsp70 chaperone protein Ssa4 tagged with GFP (Ssa4-GFP) and the metacaspase Mca1 tagged with RFP (Mca1-RFP).
The dataset was collected through fluorescence microscopy.
The image files are provided in Carl Zeiss Image format (.czi).
Data contains personal data
No
Language
Unit of analysis
Population
Saccharomyces cerevisiae (Baker's yeast)
Study design
Experimental study
Preclinical study
Sampling procedure
Time period(s) investigated
2012 – 2020
Data format / data structure
Responsible department/unit
Institute of Biomedicine, Department of Microbiology and Immunology
Research area
Biochemistry and molecular biology (Standard för svensk indelning av forskningsämnen 2011)
Cell biology (Standard för svensk indelning av forskningsämnen 2011)
Microbiology (Standard för svensk indelning av forskningsämnen 2011)
Cell and molecular biology (Standard för svensk indelning av forskningsämnen 2011)
Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, Johansson G, Gustafsson T, Kvint K, Nyström T. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. bioRxiv. 2020 Jun 29. Available from: https://www.biorxiv.org/content/10.1101/2020.06.25.170670v2.full
DOI:
https://doi.org/10.1101/2020.06.25.170670
Andersson R, Eisele-Bürger AM, Hanzén S, Vielfort K, Öling D, Eisele F, et al. (2021) Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17(1): e1008951.
DOI:
https://doi.org/10.1371/journal.pgen.1008951
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