Prokaryotic plankton respiration, growth and abundance in the Arctic Ocean 2021
SND-ID: 2023-210. Version: 1. DOI: https://doi.org/10.5878/6qa4-cn46
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Creator/Principal investigator(s)
Johan Wikner
- Umeå University, Ecology and Environmental Science
Jarone Pinhassi
- Linneaus University, Biology and Environmental science
Ashish Verma
- Umeå University, Ecology and Environmental Science
Dennis Amnebrink
- Linneaus University, Biology and Environmental Scinece
Research principal
Umeå University
- Ekologi, miljö och geovetenskap
Principal's reference number
SMK-1851
Description
The dataset consists of results from measurements of prokaryotic (Bacteria and Archaea) plankton respiration, growth, and abundance in the Arctic Ocean surface (0-500 m) in 2021. Data was collected during the Synoptic Arctic Survey expedition with the Swedish research icebreaker Oden between July 25 and September 20, 2021.
Samples were collected primarily with Niskin-bottles on a rosette-sampler equipped with a CTD probe. Some samples were also taken in the boundary layer between ice and seawater with a Ruttner sampler after a hole to the water surface was drilled.
Process speeds were measured on board within an hour of sampling in a cool laboratory with a temperature of 7°C. Plankton respiration was measured by oxygen optodes in eight simultaneous samples incubated at in situ temperature. For the PROMAC project, all samples were pre-filtered with 1.2 μm filters to specifically measure prokaryotic respiration. Growth of the prokaryotic community was measured by uptake of the DNA base thymidine labeled with tritium incubated at in situ temperature. The presence of prokaryotes was measured by
Samples were collected primarily with Niskin-bottles on a rosette-sampler equipped with a CTD probe. Some samples were also taken in the boundary layer between ice and seawater with a Ruttner sampler after a hole to the water surface was drilled.
Process speeds were measured on board within an hour of sampling in a cool laboratory with a temperature of 7°C. Plankton respiration was measured by oxygen optodes in eight simultaneous samples incubated at in situ temperature. For the PROMAC project, all samples were pre-filtered with 1.2 μm filters to specifically measure prokaryotic respiration. Growth of the prokaryotic community was measured by uptake of the DNA base thymidine labeled with tritium incubated at in situ temperature. The presence of prokaryotes was measured by epifluorescence microscopy after labeling with the dye acridine orange. Cell morphology and interactions were also studied via scanning electron microscopy in samples preserved with glutaraldehyde. In the filtered samples, the presence of different prokaryotic taxa was determined by sequencing the 16S RNA gene. In addition, expression of genes from isolated mRNA was measured. For metagenomics and transcriptomics from unfiltered samples, please refer to the OMICS project from the same expedition.
The main projects (“SASIce24h” and “SAS”) aim to contribute knowledge about prokaryotic plankton respiration, growth rate and total biomass to better understand the turnover of carbon in the Arctic Ocean and its regulation. The results from the expedition will also provide a basis for comparison with similar studies in the future to follow climate change. The spatial coverage will increase the understanding of how processes vary in different parts of the ocean. Simultaneous measurements in other parts of the Arctic Ocean by other countries will contribute to a more comprehensive picture. The “PROMAC” project studies the maintenance respiration of prokaryotic plankton and what activities are included there. The temperature sensitivity of plankton respiration is investigated in the “RespirationQ10”-project. Determination of the conversion factor from thymidine uptake to cell growth is carried out in the subproject "TCF". Whether enough of the trace element thymidine was added was investigated in the sub-project ”Isotope dilution”. First results will be published during 2024. Show less..
Data contains personal data
No
Language
Time period(s) investigated
2021-07-25 – 2021-09-20
Species and taxons
- Mode of collection: Field/Intervention experiment
- Description of the mode of collection: Collection of water samples
- Time period(s) for data collection: 2021-07-25 – 2021-09-20
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Data collector:
Umeå University
- Instrument: Water bottles - Water bottles on rosette sampler
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Sample:
Water samples
Water samples from the Arctic Ocean - Temporal resolution: 1 day
- Spatial resolution: 60 kilometres
Geographic spread
Geographic location: Arctic Ocean
Geographic description: The expedition covered the Nansen and Amundsen basins between Svalbard, the North pole and northern Greenland. Pleas refer to the Synoptic Arctic Survey expeditions report.
Responsible department/unit
Ekologi, miljö och geovetenskap
Other research principals
Funding
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Funding agency:
The Kempe foundations
- Funding agency's reference number: SMK-1854
- Project name on the application: Morfologisk och genetisk anpassning hos Arktiska bakterier vid extremt låg tillväxt
Research area
Climate research (Standard för svensk indelning av forskningsämnen 2011)
Environmental sciences (Standard för svensk indelning av forskningsämnen 2011)
Microbiology (Standard för svensk indelning av forskningsämnen 2011)
Ecology (Standard för svensk indelning av forskningsämnen 2011)
Biota (INSPIRE topic categories)
Climatology / meteorology / atmosphere (INSPIRE topic categories)
Oceans (INSPIRE topic categories)
Environment (INSPIRE topic categories)
