In this study DNA extracted from honeybees was analysed using qPCR for the presence and quantity of different honeybee-specific lactic acid bacteria. The aim was to investigate if presence of the disease American foulbrood in the beehive or in the apiary affects honeybees composition of lactic acid bacteria. In the data file "Sample_info" background information for the individual samples is found, the data file contains 7 columns and 42 rows. In the data file "Data_LAB_AFB_in_Sweden" Cq values are found for all samples and bacteria, the data file contains 11 columns and 493 rows. In the data file "Bactdilutions", bacterial concentrations and Cq values for serial dilutions of the bacteria the samples were analyzed for are found. The data file “Bactdilutions” contains 3 columns and 47 rows.

Data contains personal data

Language

English

Method and outcome

Time period(s) investigated

2018-05 – 2018-06

Data format / data structure

Numeric

Species and taxons

Apis mellifera linnaeus, 1758
Bifidobacterium asteroides scardovi & trovatelli, 1969
Bifidobacterium coryneforme (ex scardovi & trovatelli, 1969) biavati et al., 1982
Apilactobacillus apinorum
Lactobacillus kullabergensis
Bifidobacteria asteroides
Lactobacillus apis
Bombilactobacillus mellis
Lactobacillus melliventris
Bifidobacteria coryneforme
Lactobacillus helsingborgensis
Apilactobacillus kunkeei
Lactobacillus kimbladii

Data collection

Description of the mode of collection: The data was generated from qPCR analyses of DNA extracted from honeybees.
Data collector: Swedish University of Agricultural Sciences
Instrument: CFX96 Touch Real-Time PCR Detection System - qPCR ´machine from BioRad

Geographic coverage

Geographic spread

Geographic location: Sweden

Administrative information

Responsible department/unit

Department of Ecology

Contributor(s)

Paul D'Alvise - University hospital Tübingen, Institute for Clinical Microbiology and Hygiene

Eva Forsgren - Swedish University of Agricultural Sciences, Department of Ecology

Meghan Milbraith - Swedish University of Agricultural Sciences, Department of Ecology

Topic and keywords

Research area

Microbiology (Standard för svensk indelning av forskningsämnen 2011)

Keywords

Microbiome, Bacteria, Lactic acid bacteria, American foulbrood, Paenibacillus larvae

Publications

Nilsson, A., D’Alvise, P., Milbrath, M. O., & Forsgren, E. (2024). Lactic acid bacteria in Swedish honey bees during outbreaks of American foulbrood. Ecology and Evolution, 14, e10964. https://doi.org/10.1002/ece3.10964
DOI: https://doi.org/10.1002/ece3.10964

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