Phenotypic microarray data for three bacterial BCA that were exposed to six different wavelengths

SND-ID: 2024-527. Version: 1. DOI: https://doi.org/10.5878/gxa3-aa94

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SLF_420_allhours_kinetic.csv (815.24 KB)

SLF_450_allHours_kinetic.csv (1.29 MB)

SLF_530_allHours_kinetic.csv (1.29 MB)

SLF_620_allHours_kinetic.csv (1.25 MB)

SLF_660_allHours_kinetic.csv (1.29 MB)

SLF_Darkness_allHours_kinetic.csv (9.03 MB)

Associated documentation

ReadMe_dataset.txt (2.8 KB)

The power of light Impact on the perfomance of biocontrol agents under minimal nutrient conditions.pdf (2.37 MB)

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2024-527-1.zip (~17.32 MB)

Citation

Creator/Principal investigator(s)

Maria Karlsson - Swedish University of Agricultural Sciences, Department of Biosystems and Technology

Maria Hellström - Swedish University of Agricultural Sciences, Department of Biosystems and Technology

Swedish University of Agricultural Sciences, Department of Biosystems and Technology

Research principal

Swedish University of Agricultural Sciences - Department of Biosystems and Technology

Principal's reference number

SLU.ltv.2024.4.4.IÄ-16

Description

Phenotypic microarray data (PM 1 and PM 2) involving three bacterial strains, Bacillus amyloliquefaciens DSM7, Pseudomonas chlororaphis 58003, Streptomyces griseoviridis CBS904.68, under six light treatments (Darkness, 420, 460, 530, 630, 660 nm at 50 μmol per m2 per second) with six replicates for each. Each csv file is the raw data for one light treatment and all three bacteria. The output is in OmniLog values, which were measured as a colour change of tetrazolium blue due to respiration. Measurements for the dark treatment occurred every 15 min for 96 hours. For the other treatments manual measurements were taken every: 0, 6, 10, 24, 30, 48, 54, 72, and 96 h post inoculation (hpi). Measurements for the light treatments in the OmniLog incubator took one hour. For more information, please see the README file.

Data contains personal data

Language

English

Method and outcome

Unit of analysis

Other

Population

Six replicates of three bacterial strains were exposed to different wavelengths for 96 hours. Readings were set to occur 0, 6, 10, 24, 30, 48, 54, 72, and 96 h post-inoculation (hpi). Output values were expressed in OmniLog units. The darkness treatment had readings every 15 minutes throughout the 96 hours.

Time Method

Time series

Other

Sampling procedure

Other

Under dark conditions, the colour change was recorded automatically every 15 min, while under light exposure, the colour change of the PM panels was recorded according to previously established growth curves. Readings were set to occur 0, 6, 10, 24, 30, 48, 54, 72, and 96 hours post-inoculation (hpi). Output values were expressed in OmniLog units.

Number of individuals/objects

108

Data format / data structure

Numeric

Data collection

Mode of collection: Experiment
Description of the mode of collection: Phenotypic microarrays (PM) were performed following procedures described by Gharaie et al. (2017) and Alsanius et al. (2021).
Data collector: Swedish University of Agricultural Sciences
Instrument: Omnilog (Technical instrument(s)) - Machine used for microbial phenotypic characterization and identification.
Source of the data: Research data, Research data: Published

Geographic coverage

Administrative information

Responsible department/unit

Department of Biosystems and Technology

Contributor(s)

Beatrix Alsanius - Swedish University of Agricultural Sciences, Department of Biosystems and Technology

Swedish University of Agricultural Sciences

Funding

Funding agency: Swedish farmers’ foundation for agricultural research
Funding agency's reference number: R-18-25-006
Project name on the application: Optimized integrated control in greenhouse systems sees the light

Topic and keywords

Research area

Microbiology (Standard för svensk indelning av forskningsämnen 2011)

Horticulture (Standard för svensk indelning av forskningsämnen 2011)

Keywords

Phenotypic microarray, Biocontrol agents, Sole-carbon source utlisation, Biofilm formation, Light quality, Wavelength

Publications

Karlsson, M. E., Hellström, M., Flöhr, A., Bergstrand, K.-J., & Alsanius, B. W. (2023). The power of light: Impact on the performance of biocontrol agents under minimal nutrient conditions. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1087639
DOI: https://doi.org/10.3389/fmicb.2023.1087639

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