CG4928 knockdown affects the expression of membrane-bound, biosynthesis and signaling genes : RNA sequencing data from CG4928 knockdown flies and controls

Mikaela M. Ceder - Uppsala University, Department of Pharmaceutical Biosciences

Malpighian tubules from 3-day-old females (da-Gal4 > CG4928 RNAi, da-Gal4 > w1118, and w1118 > CG4928 RNAi) were dissected, pooled, and frozen in −80°C until analysis. A total of three replicates per genotype were collected, each replicate containing 60 Malpighian tubules. Analysis was performed using inductive coupled plasma sector field mass spectrometry (ICP-SFMS) to analyze a total of 69 elements (ions) in each sample. A control sample was used to measure background in the collecting media. The data were within group, normalized using geometric mean before means (±SD) were calculated and outliers were removed. GraphPad Prism, version 5, was used to perform one-way ANOVA using unpaired t-test with Bonferroni’s multiple correction (adjusted p-values: ∗p < 0.0492, ∗∗p < 0.0099, ∗∗∗p < 0.0001). The observed data points for boron, cadmium, cobalt, and potassium, which were the only elements found to be altered, are plotted using scatter plot, where the line displays the mean value.

The dataset was originally published in DiVA and moved to SND in 2024.

English