Unique brood ester profile in a Varroa destructor resistant population of European honey bee (Apis mellifera)

SND-ID: 2024-173. Version: 1. DOI: https://doi.org/10.5878/h2hc-h513


Creator/Principal investigator(s)

Nicholas Scaramella - Swedish University of Agricultural Sciences, Department of Ecology orcid

Barbara Locke - Swedish University of Agricultural Sciences, Department of Ecology orcid

Research principal

Swedish University of Agricultural Sciences - Department of Ecology rorId

Principal's reference number



Data of chemical compounds extracted from honey bee (Apis mellifera) brood in a varroa resistant and varroa susceptible population. Samples were taken 0, 6, 12, 18, 24, and 36 hours after brood capping by immersing pupae in pentane for 10 minutes. 6 hives from each population were used, and 3 replicates were taken per hive per time point. The FAME column was calculated by adding Methyl Palmitate, Linoloate, and Stearate together, while the FAEE columb was calculateed by adding Ethyl Palmitate, Linoloate, and Stearate together. Samples were analyzed using gas chromatography.

R version 4.4.0 and RStudio version were used to analyze the data. All packages and their version is listed in the attached R script.

Data files included:
Scaramella_et_al_2024_Contrast.tsv: 16 rows × 12 columns
Scaramella_et_al_2024_Data.tsv: 210 rows × 14 columns

Data contains personal data



Method and outcome

Time period(s) investigated

2019-06 – 2019-08

Data format / data structure

Data collection
  • Mode of collection: Physical measurements and tests
  • Description of the mode of collection:
    Pupae at pre-designated times (0, 6, 12, 18, 36 hours after brood capping) were removed and submerged in n-pentane for 10 minutes to remove voltile and semi-voltile compounds from the cuticular.
    These compounds were analysed using gas chromatography.
    Full description of methods available in manuscript.
  • Time period(s) for data collection: 2021-06 – 2021-08
  • Data collector: Swedish University of Agricultural Sciences
  • Instrument: Gas Chromatography - A machine that vaporizes compounds, which flows along a long tube, with different chemicals in the compound reaching a sensor at different times. By comparing to documented times it takes a chemical to reach the sensor, chemicals can be quantified
  • Sample: Brood cuticular compounds
    Cuticular compounds extracted from Apis mellifera pupae in n-pentane
  • Source of the data: Research data: Unpublished, Research data
  • Temporal resolution: 3 month
  • Spatial resolution: 750 metres
Geographic coverage
Administrative information

Responsible department/unit

Department of Ecology


  • Funding agency: European Research Council rorId
  • Funding agency's reference number: 949223
  • Project name on the application: ERC Starting Grant
Topic and keywords

Research area

Zoology (Standard för svensk indelning av forskningsämnen 2011)

Ecology (Standard för svensk indelning av forskningsämnen 2011)

Evolutionary biology (Standard för svensk indelning av forskningsämnen 2011)



Version 1. 2024-06-24

Version 1: 2024-06-24

DOI: https://doi.org/10.5878/h2hc-h513

Contacts for questions about the data

Nicholas Scaramella


SLU Arkiv


Published: 2024-06-24