A landscape of X-inactivation during human T-cell development in the thymus

SND-ID: 2022-112-1. Version: 1. DOI: https://doi.org/10.5878/frw5-wd59


Creator/Principal investigator(s)

Olov Ekwall - University of Gothenburg, Institute of Clinical Sciences, Department of Pediatrics orcid

Research principal

University of Gothenburg - Institute of Clinical Sciences rorId


The aim of the study was to investigate X-inactivation during human T-cell development in the thymus. Thymocytes from six different developmental stages from seven individuals were isolated and studied regarding gene expression. The data set contains data from RNA sequencing, DNA methylation and whole exome sequencing.

The dataset contains data from the manuscript "A landscape of X-inactivation during human T-cell development in the thymus" by Gylemo et al, and consists of results from RNA sequencing (fastq.gz), analysis of DNA methylation (Grn.idat) and whole exome DNA sequencing (fastq.gz) of six developmental stages of thymocytes from seven individuals.

The data is described on individual and cell-type level in the tab-separated metadata files (.tsv).
MD5 checksums for each file are included.

Data contains personal data


Sensitive personal data


Type of personal data

Human data. The data is pseudonymized and contains ID-numbers that can be coupled to individuals through a code-key.

Code key exists



Method and outcome

Unit of analysis


Isolated thymocytes from seven persons were studied, three boys and four girls, all under 3 yrs that underwent cardiac surgery for correction of congenital heart defects.

Study design

Experimental study

Sampling procedure

Biobank is connected to the study

The study has collected samples/material which are stored in a scientific collection or biobank

Scientific collection or biobank name: Biobank Väst 890

Type(s) of sample: Isolerade thymocyter

This study has used existing samples from a scientific collection or biobank

Number of individuals/objects


Data format / data structure

Data collection
Geographic coverage

Geographic spread

Geographic location: Sweden

Administrative information

Responsible department/unit

Institute of Clinical Sciences

Other research principals


Colm E Nestor - Linköping University, Department of Biomedical and Clinical Sciences, Division of Children's and Women's Health orcid

Funding 1

  • Funding agency: The ALF agreement: The Swedish state under the ALF agreement between the Swedish government and the county councils
  • Funding agency's reference number: ALFGBG-965795
  • Project name on the application: Kliniska och experimentella studier av toleransmekanismer i thymus

Funding 2

  • Funding agency: Swedish Research Council rorId
  • Funding agency's reference number: 2018-02752
  • Project name on the application: Clinical and experimental studies of tolerance mechanisms in the thymus aiming at the development of therapeutic targeting of the thymus in the treatment of autoimmune diseases

Ethics Review

Gothenburg - Ref. 217-12

Project title: Studies of central tolerance induction in human thymus

Topic and keywords

Research area

Immunology in the medical area (Standard för svensk indelning av forskningsämnen 2011)


Björn Gylemo, Antonio Lentini, Christina Lundqvist, Maike Bensberg, Alessandro Camponeschi, Dora Goldman, Aida Selimović, Olov Ekwall, Colm E. Nestor. (2022). A landscape of X-inactivation during human T-cell development [submitted manuscript].

If you have published anything based on these data, please notify us with a reference to your publication(s). If you are responsible for the catalogue entry, you can update the metadata/data description in DORIS.

Published: 2022-09-06
Last updated: 2022-09-21